Electrodes for In Vivo Electroporation

Ideal electrode for electroporation of mouse fertilized eggs for the creation of genome-edited mice. (CUY21EDIT II, Genome Editor compliant electrode)

Intended transfection site: mouse fertilized egg

Fixed distance between electrodes makes this electrode suitable for routine work.

Intended site of introduction: Ectoderm-derived tissue of chick embryos (e.g. neural tube)

This electrode is suitable for electroporation to the ectoderm of chick embryos.　For electroporation of mesodermal parts such as body segments, use in combination with the LF613 series electrodes.

Intended site of introduction: mesoderm and ectoderm derived tissues (neural tube, etc.) of the chick embryo

Shaped for easy placement under the chick embryo.　It is ideal for electroporation of the endoderm and mesoderm of the chick embryo and should be used in combination with the LF611 or LF614 series electrodes.

Target site: endoderm and mesoderm derived tissues of chick embryos (digestive organs, heart, etc.)

Can be attached to micromanipulators.　

Ideal electrode for localized electroporation.　The LF614S is insulated and coated except for the 1mm tip to minimize damage to the embryo.　It is ideal for electroporation of the heart and other parts of the chicken embryo.

Intended site of introduction: local area of ectoderm, endoderm, or mesoderm of chick embryo

Comes with a 75mm long cable.　Can be attached to micromanipulators.　

The tip diameter of the electrode is extremely narrow, making it ideal for electroporation of African clawed frog embryos.　

Intended introduction site: African clawed frog embryo

The LF200S can be used as both an electrode for electroporation and a needle for DNA buffer injection.　By connecting a micro syringe pump to the electrode, DNA buffer can be injected.　This reduces complexity and damage to the mouse brain.

Intended site of transfection: Adult mouse brain

The LF200-1.0 is a new type of electrode that solves the problems of the LF200S with needle insertion and DNA buffer injection. Both electrodes are now integrated into a single unit, so that only a single needle is needed to be inserted. It is less complicated and causes much less damage to the mouse brain.

Intended site of introduction: Adult mouse brain, chicken embryo (E9)

This is an electrode with two needles fixed in parallel.　It can be inserted into the target area, such as a muscle, for efficient operation.

Target sites of introduction: muscles of mice and rats, brains of neonatal rats, etc.

The distance between two parallel needles can be varied for a wide range of applications.

Target sites of introduction: honeybee brain, chick terminal brain, etc.

A rectangular (square) electrode is attached to the tip of the tweezers.　Ideal for long, thin samples such as pancreas, genitalia, etc.

Target sites: pancreas of mice and rats, lungs of rats, prostate of rats, reproductive organs of black sea bream, etc.

A circular electrode is attached to the tip of the tweezers.　Various sizes are available.　Can be used for a wide range of applications from fetal brain in utero to organs in adult mice. Versatile electrodes.

Target sites: skin and organs (liver, testis, etc.) of mice and rats, retina of neonatal mice and rats, brain of intrauterine fetuses, inner ear of intrauterine fetuses, etc.

Circular electrodes of different diameters are attached to the tip of the tweezers.　The electrode on the anode (+) side, where DNA and RNA are intensively introduced, is smaller than the electrode on the cathode (-) side, making it ideal for local electroporation.　

Target sites for introduction: localized areas such as organs of mice and rats, and fetal brain in utero

U-shaped to match the shape of the fetal mouse spinal cord.

Intended site of introduction: fetal mouse spinal cord

Fork (needle) type and rectangular type electrodes are attached to the tip of the tweezers.　The fork electrode can be inserted under the skin and the rectangular electrode can be inserted between the skin and the fork electrode, making it ideal for electroporation of the skin (tumors).

Intended site of introduction: mouse, rat, and human skin

Two platinum electrodes are mounted inside a small chamber.　Ideal for electroporation of excised organs, tissues, and fetal mice.

Intended introduction sites: sections of the anterior stomach of a chicken embryo, sections of the small intestine of a mouse, mouse fetuses, harvested blood vessels, etc.

An electrode with a circular electrode attached to the center of the petri dish and the tip of the rod.　Ideal for electroporation of tissue sections, chicken embryos, African clawed frog embryos, etc., by placing them between the top and bottom.

Intended introduction sites: mouse and rat tissue sections (e.g. hippocampal sections), early chick embryos, African clawed frog embryos, etc.

This electrode has a square electrode attached to the center of the petri dish and the tip of the rod.　Ideal for electroporation of tissue sections, chicken embryos, etc. by placing them between the top and bottom.　

Intended introduction sites: mouse and rat tissue sections (e.g., hippocampal sections), early chick embryos, etc.

We will fabricate a holder to fit your glass capillary and micromanipulator rods.　

For: single cells (adipocytes, plant cells)

Allows electroporation in culture on a dish.　The 0.5mm high feet around the electrode prevent the electrode from directly touching the cells, thus reducing damage to the cells. \The electrode tip is removable and the tip can be autoclaved.

Transduced Cells: Primary Transgenic Neurons

The polarity of the electroporation can be switched.　Since DNA and RNA are concentrated on the anode side, the introduction range can be greatly expanded by reversing the polarity between the electrodes.

The D200 Foot Switch allows you to step on the switch with your foot to execute electroporation programs even if you are unable to use both hands.